Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behaviour.Ontological description: MP:0005266 - abnormal metabolism.

The Open Field test is used to assess anxiety and exploratory behaviors. It is based on the natural tendency of an animal to explore and to protect itself using avoidance which translates to a normal animal spending more time in the periphery of the Open Field arena than in the center (the most anxiogenic area).

Use modified SHIRPA for a simple first-line phenotyping screen.

The energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system.Very precise CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and finally, heat production can be calculated. An activity and food intake monitoring system can also be integrated to the set up for the measurement of activity e.g. to ensure that periods of resting can be identified.

Light-Dark test monitors the anxiety-related behaviour of mice

The Open Field test is used to assess anxiety and exploratory behaviors. It is based on the natural tendency of an animal to explore and to protect itself using avoidance which translates to a normal animal spending more time in the periphery of the Open Field arena than in the center (the most anxiogenic area).DescriptionMP Term or Commentabnormal anxiety-related reponseMP:0001362abnormal locomoter activityMP:0001392abnormal response to novel environmentMP:0001449&nbsp;

Dark-Light test monitors the anxiety-related behaviour of mice

The Open Field test is used to assess anxiety and exploratory drive. It is based on the natural tendency of an animal to explore and avoidance reaction to protect itself, which make normal animals to spend more time in the corners and the periphery than in the centre (the most anxiogenic area).

To monitor the anxiety-related behavior of mice in a two-chambered light-dark arena over a defined period of time in order to assess the anxiety phenotype. 

Y-maze assesses learning and memory; it can be used instead/in parallel of Fear Conditioning



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My Genes

decreased locomotor activity

Description

IMPC Gene variants with decreased locomotor activity

Most significant associations for decreased locomotor activity

Associations details of selected gene(s):

The way we measure

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