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The EUCOMM program

EUCOMM – The European Conditional Mouse Mutagenesis Program is founder member and European cornerstone of the International Knockout Mouse Consortium (IKMC).
EUCOMM contributes the largest fraction of conditionally trapped and targeted genes in mouse C57BL/6N embryonic stem (ES) cells to the IKMC.
EUCOMM vectors, mutant ES cells and mutant mice are distributed worldwide, enabling functional genomics research in a standardized and cost-effective manner by a much wider biomedical research community than has been possible previously.
EUCOMM mutant ES cells and vectors can be obtained from the European Mouse Mutant Cell Repository (EuMMCR).
EUCOMM mutant mice are archived and distributed by the European Mouse Mutant Archive (EMMA).

EUCOMM Farewell Meeting Participants

EUCOMM Project Information

Main Objectives

• Generation, archiving, and world-wide distribution of up to 12.000 conditional mutations across the mouse genome in mouse embryonic stem (ES) cells
• Establishment of a limited number of mouse mutants from this resource

Mutagenesis Strategies

• Conditional gene trapping – random approach for expressed genes
• Conditional targeted trapping – directed approach, used for expressed genes
• Conditional gene targeting – directed approach, used for non-expressed genes

Networking

• Generation of European mouse functional genomics platform by synergistic integration of EMMA, EUMODIC, EURExpress, FLPFLEX ,FunGenES, PRIME and others
• Cornerstone of world-wide mouse mutagenesis effort, member of the International Knockout Mouse Consortium (IKMC)

Societal Benefits

• Improvement of health research
• Speedup of drug development, therapy and diagnosis
• Competitive advantage for pharmaceutical industry

Funding & Structure

• Funded by European Union (FP 6, Priority 1 “Life Sciences, Genomics and Biotechnology for Health”)
• EU funding: up to 13 million Euros
• Integrated Project
• Consortium: 9 participants from four European countries
 

EUCOMM Scientific Advisory Board

  • Phil Avner, Paris, France
  • David Beier, Boston, MA, USA
  • Pierre Chambon, Strasbourg, France
  • Deanna Church, Bethesda, MD, USA
  • Frank Grosveld, Rotterdam, The Netherlands
  • Monica Justice, Houston, TX, USA
  • George Kollias, Vari, Greece
  • Björn Löwenadler, Mölndal, Sweden
  • Bernard Malissen, Marseille-Luminy, France
  • Andrew McMahon, Cambridge, MA, USA
  • H. Earl Ruley, Nashville, TN, USA
  • Phil Soriano, New York, NY, USA

EUCOMM Coordination

  • Wolfgang Wurst
  • Allan Bradley

EUCOMM Management

  • Cornelia Kaloff
  • Derek Matthews
  • Karin Simmerl

EUCOMM Participants

EUCOMM Publications

2012

  • Highly-efficient, fluorescent, locus directed cre and FlpO deleter mice on a pure C57BL/6N genetic background.
    Birling MC, Dierich A, Jacquot S, Hérault Y, Pavlovic G.
    Genesis. 2012 Jun;50(6):482-9. PMID: 22121025 DOI: 10.1002/dvg.20826
  • The mammalian gene function resource: the international knockout mouse consortium.
    Bradley A, Anastassiadis K, Ayadi A, Battey JF, Bell C, Birling MC, Bottomley J, Brown SD, Bürger A, Bult CJ, Bushell W, Collins FS, Desaintes C, Doe B, Economides A, Eppig JT, Finnell RH, Fletcher C, Fray M, Frendewey D, Friedel RH, Grosveld FG, Hansen J, Hérault Y, Hicks G, Hörlein A, Houghton R, Hrabé de Angelis M, Huylebroeck D, Iyer V, de Jong PJ, Kadin JA, Kaloff C, Kennedy K, Koutsourakis M, Lloyd KCK, Marschall S, Mason J, McKerlie C, McLeod MP, von Melchner H, Moore M, Mujica AO, Nagy A, Nefedov M, Nutter LM, Pavlovic G, Peterson JL, Pollock J, Ramirez-Solis R, Rancourt DE, Raspa M, Remacle JE, Ringwald M, Rosen B, Rosenthal N, Rossant J, Ruiz Noppinger P, Ryder E, Schick JZ, Schnütgen F, Schofield P, Seisenberger C, Selloum M, Simpson EM, Skarnes WC, Smedley D, Stanford WL, Stewart AF, Stone K, Swan K, Tadepally H, Teboul L, Tocchini-Valentini GP, Valenzuela D, West AP, Yamamura KI, Yoshinaga Y, Wurst W.
    Mamm Genome. 2012 Oct;23(9-10):580-6. PMID: 22968824 DOI: 10.1007/s00335-012-9422-2
  • Isolation of homozygous mutant mouse embryonic stem cells using a dual selection system.
    Huang Y, Pettitt SJ, Guo G, Liu G, Li MA, Yang F, Bradley A.
    Nucleic Acids Res. 2012 Feb 1;40(3):e21. PMID: 22127858 DOI: 10.1093/nar/gkr908

2011

  • High efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes.
    Bird AW, Erler A, Fu J, Hériché JK, Maresca M, Zhang Y, Hyman AA, Stewart AF.
    Nat Methods. 2011 Dec 4;9(1):103-9. PMID: 22138824 DOI: 10.1038/nmeth.1803
  • Disease model discovery and translation. Introduction.
    Brown SD.
    Mamm Genome. 2011 Aug;22(7-8):361. PMID: 21796466 DOI: 10.1007/s00335-011-9352-4
  • HIF-VEGF pathways are critical for chronic otitis media in Junbo and Jeff mouse mutants.
    Cheeseman MT, Tyrer HE, Williams D, Hough TA, Pathak P, Romero MR, Hilton H, Bali S, Parker A, Vizor L, Purnell T, Vowell K, Wells S, Bhutta MF, Potter PK, Brown SD.
    PLoS Genet. 2011 Oct;7(10):e1002336. PMID: 22028672 DOI: 10.1371/journal.pgen.1002336
  • Genetic screens using the piggyBac transposon.
    Chew SK, Rad R, Futreal PA, Bradley A, Liu P.
    Methods. 2011 Apr;53(4):366-71. PMID: 21185377 DOI: 10.1016/j.ymeth.2010.12.022
  • Recombineering BAC transgenes for protein tagging.
    Ciotta G, Hofemeister H, Maresca M, Fu J, Sarov M, Anastassiadis K, Stewart AF.
    Methods. 2011 Feb;53(2):113-9. PMID: 20868752 DOI: 10.1016/j.ymeth.2010.09.003
  • Disruption of mouse Slx4, a regulator of structure-specific nucleases, phenocopies Fanconi anemia.
    Crossan GP, van der Weyden L, Rosado IV, Langevin F, Gaillard PH, McIntyre RE; Sanger Mouse Genetics Project, Gallagher F, Kettunen MI, Lewis DY, Brindle K, Arends MJ, Adams DJ, Patel KJ
    Nat Genet. 2011 Feb;43(2):147-52. PMID: 21240276 DOI: 10.1038/ng.752
  • Recombination-mediated genetic engineering of large genomic DNA transgenes.
    Ejsmont RK, Ahlfeld P, Pozniakovsky A, Stewart AF, Tomancak P, Sarov M.
    Methods Mol Biol. 2011;772:445-58. PMID: 22065454 DOI: 10.1007/978-1-61779-228-1_26
  • Forward and reverse genetics through derivation of haploid mouse embryonic stem cells.
    Elling U, Taubenschmid J, Wirnsberger G, O’Malley R, Demers SP, Vanhaelen Q, Shukalyuk AI, Schmauss G, Schramek D, Schnuetgen F, von Melchner H, Ecker JR, Stanford WL, Zuber J, Stark A, Penninger JM.
    Cell Stem Cell. 2011 Dec 2;9(6):563-74. PMID: 22136931 DOI: 10.1016./j.stem.2011.10.012
  • Generating conditional knockout mice.
    Friedel RH, Wurst W, Wefers B, Kühn R
    Methods Mol Biol. 2011;693;205-31. PMID: 21080282 DOI: 10.1007/978-1-60761-974-1_12
  • Mouse phenotyping.
    Fuchs H, Gailus-Durner V, Adler T, Aguilar-Pimentel JA, Becker L, Calzada-Wack J, Da Silva-Buttkus P, Neff F, Götz A, Hans W, Hölter SM, Horsch M, Kastenmüller G, Kemter E, Lengger C, Maier H, Matloka M, Möller G, Naton B, Prehn C, Puk O, Rácz I, Rathkolb B, Römisch-Margl W, Rozman J, Wang-Sattler R, Schrewe A, Stöger C, Tost M, Adamski J, Aigner B, Beckers J, Behrendt H, Busch DH, Esposito I, Graw J, Illig T, Ivandic B, Klingenspor M, Klopstock T, Kremmer E, Mempel M, Neschen S, Ollert M, Schulz H, Suhre K, Wolf E, Wurst W, Zimmer A, Hrabé de Angelis M.
    Methods. 2011 Feb;53(2):120-35. Review. PMID: 20708688 DOI: 10.1016/j.ymeth.2010.08.006
  • High-throughput mouse phenotyping.
    Gates H, Mallon AM, Brown SD; EUMODIC Consortium.
    Methods. 2011 Apr;53(4):394-404. PMID: 21185382 DOI: 10.1016/j.ymeth.2010.12.017
  • BioMart Central Portal: an open database network for the biological community.
    Guberman JM, Ai J, Arnaiz O, Baran J, Blake A, Baldock R, Chelala C, Croft D, Cros A, Cutts RJ, Di Génova A, Forbes S, Fujisawa T, Gadaleta E, Goodstein DM, Gundem G, Haggarty B, Haider S, Hall M, Harris T, Haw R, Hu S, Hubbard S, Hsu J, Iyer V, Jones P, Katayama T, Kinsella R, Kong L, Lawson D, Liang Y, Lopez-Bigas N, Luo J, Lush M, Mason J, Moreews F, Ndegwa N, Oakley D, Perez-Llamas C, Primig M, Rivkin E, Rosanoff S, Shepherd R, Simon R, Skarnes B, Smedley D, Sperling L, Spooner W, Stevenson P, Stone K, Teague J, Wang J, Wang J, Whitty B, Wong DT, Wong-Erasmus M, Yao L, Youens-Clark K, Yung C, Zhang J, Kasprzyk A.
    Database (Oxford). 2011 Sep 18;2011:bar041. PMID: 21930507 DOI: 10.1093/database/bar041
  • Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis.
    Hofemeister H, Ciotta G, Fu J, Seibert PM, Schulz A, Maresca M, Sarov M, Anastassiadis K, Stewart AF.
    Methods. 2011 Apr;53(4):437-52. PMID: 21195765 DOI: 10.1016/j.ymeth.2010.12.026
  • A homozygous mutant embryonic stem cell bank applicable for phenotype-driven genetic screening.
    Horie K, Kokubu C, Yoshida J, Akagi K, Isotani A, Oshitani A, Yusa K, Ikeda R, Huang Y, Bradley A, Takeda J.
    Nat Methods. 2011 Oct 23;8(12):1071-7. PMID: 22020066 DOI: 10.1038/nmeth.1739
  • Technical approaches for mouse models of human disease.
    Justice MJ, Siracusa LD, Stewart AF.
    Dis Model Mech. 2011 May;4(3):305-10. Review. PMID: 21558063 DOI: 10.1242/dmm.000901
  • Conditional RNAi in mice.
    Kleinhammer A, Deussing J, Wurst W, Kühn R.
    Methods. 2011 Feb;53(2):142-50. Review. PMID: 20705138 DOI: 10.1016/j.ymeth.2010.08.003
  • Constitutive and conditional RNAi transgenesis in mice.
    Kleinhammer A, Wurst W, Kühn R.
    Methods. 2011 Apr;53(4):430-6. PMID: 21184828 DOI: 10.1016/j.ymeth.2010.12.015
  • Animal models for arthritis: innovative tools for prevention and treatment.
    Kollias G, Papadaki P, Apparailly F, Vervoordeldonk MJ, Holmdahl R, Baumans V, Desaintes C, Di Santo J, Distler J, Garside P, Hegen M, Huizinga TW, Jüngel A, Klareskog L, McInnes I, Ragoussis I, Schett G, Hart B, Tak PP, Toes R, van den Berg W, Wurst W, Gay S.
    Ann Rheum Dis. 2011 Aug;70(8):1357-62. Review. PMID: 21628308 DOI: 10.1136/ard.2010.148551
  • Fancd2 counteracts the toxic effects of naturally produced aldehydes in mice. 
    Langevin F, Crossan GP, Rosado IV, Arends MJ, Patel KJ. 
    Nature. 2011 Jul 6;475(7354):53-8. PMID: 21734703 DOI: 10.1038/nature10192
  • Crafting rat genomes with zinc fingers.
    Li MA, Bradley A
    Nat Biotechnol. 2011;29;39-41. PMID: 21221099 DOI: 10.1038/nbt.1749
  • Mobilization of giant piggyBac transposons in the mouse genome.
    Li MA, Turner DJ, Ning Z, Yusa K, Liang Q, Eckert S, Rad L, Fitzgerald TW, Craig NL, Bradley A.
    Nucleic Acids Res. 2011 Dec;39(22):e148. PMID: 21948799 DOI: 10.1093/nar/gkr764
  • Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar development.
    Maier V, Jolicoeur C, Rayburn H, Takegahara N, Kumanogoh A, Kikutani H, Tessier-Lavigne M, Wurst W, Friedel RH.
    Mol Cell Neurosci. 2011 Feb;46(2):419-31. PMID: 21122816 DOI: 10.1016/j.mcn.2010.11.005
  • Methods for extracting function from mammalian genomes.
    von Melchner H, Stewart AF.
    Methods. 2011 Apr;53(4):329-30. PMID: 21392581 DOI: 10.1016/j.ymeth.2011.03.002
  • Aspartoacylase-LacZ Knockin Mice: An Engineered Model of Canavan Disease.
    Mersmann N, Tkachev D, Jelinek R, Röth PT, Möbius W, Ruhwedel T, Rühle S, Weber-Fahr W, Sartorius A, Klugmann M
    PLoS One. 2011;6(5):e20336. PMID: 21625469 DOI: 10.1371/journal.pone.0020336
  • Cop1 constitutively regulates c-Jun protein stability and functions as a tumor suppressor in mice. 
    Migliorini D, Bogaerts S, Defever D, Vyas R, Denecker G, Radaelli E, Zwolinska A, Depaepe V, Hochepied T, Skarnes WC, Marine JC
    J Clin Invest. 2011 Apr 1;121(4):1329-43. PMID: 21403399 DOI: 10.1172/JCI45784
  • Targeted isolation of cloned genomic regions by recombineering for haplotype phasing and isogenic targeting.
    Nedelkova M, Maresca M, Fu J, Rostovskaya M, Chenna R, Thiede C, Anastassiadis K, Sarov M, Stewart AF.
    Nucleic Acids Res. 2011 Nov 1;39(20):e137. PMID: 21852329 DOI: 10.1093/nar/gkr668
  • BioMart as an integration solution for the International Knockout Mouse Consortium.
    Oakley DJ, Iyer V, Skarnes WC, Smedley D.
    Database (Oxford). 2011 Sep 18;2011:bar028. PMID: 21930503 DOI: 10.1093/database/bar028
  • Expression of the splicing factor gene SFRS10 is reduced in human obesity and contributes to enhanced lipogenesis.
    Pihlajamäki J, Lerin C, Itkonen P, Boes T, Floss T, Schroeder J, Dearie F, Crunkhorn S, Burak F, Jimenez-Chillaron JC, Kuulasmaa T, Miettinen P, Park PJ, Nasser I, Zhao Z, Zhang Z, Xu Y, Wurst W, Ren H, Morris AJ, Stamm S, Goldfine AB, Laakso M, Patti ME.
    Cell Metab. 2011 Aug 3;14(2):208-18. PMID: 21803291 DOI: 10.1016/j.cmet.2011.06.007
  • A resource of vectors and ES cells for targeted deletion of microRNAs in mice.
    Prosser HM, Koike-Yusa H, Cooper JD, Law FC, Bradley A.
    Nat Biotechnol. 2011 Aug 7;29(9):840-5. PMID: 21822254 DOI: 10.1038/nbt.1929
  • Glutamatergic and dopaminergic neurons mediate anxiogenic and anxiolytic effects of CRHR1.
    Refojo D, Schweizer M, Kuehne C, Ehrenberg S, Thoeringer C, Vogl AM, Dedic N, Schumacher M, von Wolff G, Avrabos C, Touma C, Engblom D, Schütz G, Nave KA, Eder M, Wotjak CT, Sillaber I, Holsboer F, Wurst W, Deussing JM.
    Science. 2011 Sep 30;333(6051):1903-7. PMID: 21885734 DOI: 10.1126/science.1202107
  • The IKMC web portal: a central point of entry to data and resources from the International Knockout Mouse Consortium.
    Ringwald M, Iyer V, Mason JC, Stone KR, Tadepally HD, Kadin JA, Bult CJ, Eppig JT, Oakley DJ, Briois S, Stupka E, Maselli V, Smedley D, Liu S, Hansen J, Baldock R, Hicks GG, Skarnes WC
    Nucleic Acids Res. 2011;39;D849-55. PMID: 20929875 DOI: 10.1093/nar/gkq879
  • Unraveling the genetics of otitis media: from mouse to human and back again.
    Rye MS, Bhutta MF, Cheeseman MT, Burgner D, Blackwell JM, Brown SD, Jamieson SE.
    Mamm Genome. 2011 Feb;22(1-2):66-82. PMID: 21107580 DOI: 10.1007/s00335-010-9295-1
  • Resources for proteomics in mouse embryonic stem cells.
    Schnütgen F, Ehrmann F, Poser I, Hubner NC, Hansen J, Floss T, deVries I, Wurst W, Hyman A, Mann M, von Melchner H.
    Nat Methods. 2011 Feb;8(2):103-4. PMID: 21278719 DOI: 10.1038/nmeth0211-103
  • High throughput gene trapping and postinsertional modifications of gene trap alleles.
    Schnütgen F, Ehrmann F, Ruiz-Noppinger P, von Melchner H.
    Methods. 2011 Apr;53(4):347-55. PMID: 21334922 DOI: 10.1016/j.ymeth.2010.12.037
  • A conditional knockout resource for the genome-wide study of mouse gene function. 
    Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A
    Nature. 2011 Jun 15;474(7351):337-42. PMID: 21677750 DOI: 10.1038/nature10163
  • Cre recombinase resources for conditional mouse mutagenesis.
    Smedley D, Salimova E, Rosenthal N.
    Methods. 2011 Apr;53(4):411-6. PMID: 21195764 DOI: 10.1016/j.ymeth.2010.12.027
  • Utilization of a mutagenesis screen to generate mouse models of hyperaldosteronism.
    Spyroglou A, Wagner S, Gomez-Sanchez C, Rathkolb B, Wolf E, Manolopoulou J, Reincke M, Bidlingmaier M, Hrabé de Angelis M, Beuschlein F.
    Endocrinology. 2011 Jan;152(1):326-31. PMID: 21068155 DOI: 10.1210/en.2010-1081
  • Bi-allelic gene targeting in mouse embryonic stem cells. 
    Tate PH, Skarnes WC. 
    Methods. 2011 Apr;53(4):331-8. PMID: 21288739 DOI: 10.1016/j.ymeth.2010.12.025
  • Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog 1.
    Wang W, Yang J, Liu H, Lu D, Chen X, Zenonos Z, Campos LS, Rad R, Guo G, Zhang S, Bradley A, Liu P.
    Proc Natl Acad Sci U S A. 2011 Nov 8;108(45):18283-8. PMID: 21990348 DOI: 10.1073/pnas.1100893108
  • Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells.
    Yusa K, Rashid ST, Strick-Marchand H, Varela I, Liu PQ, Paschon DE, Miranda E, Ordóñez A, Hannan NR, Rouhani FJ, Darche S, Alexander G, Marciniak SJ, Fusaki N, Hasegawa M, Holmes MC, Di Santo JP, Lomas DA, Bradley A, Vallier L.
    Nature. 2011 Oct 12;478(7369):391-4. PMID: 21993621 DOI: 10.1038/nature10424
  • A hyperactive piggyBac transposase for mammalian applications.
    Yusa K, Zhou L, Li MA, Bradley A, Craig NL
    Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1531-6. PMID: 21205896 DOI: 10.1073/pnas.1008322108

2010

  • A practical summary of site-specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT2.
    Anastassiadis K, Glaser S, Kranz A, Berhardt K, Stewart AF
    Methods Enzymol. 2010;477;109-23. PMID: 20699139 DOI: 10.1016/S0076-6879(10)77007-5
  • MouseBook: an integrated portal of mouse resources.
    Blake A, Pickford K, Greenaway S, Thomas S, Pickard A, Williamson CM, Adams NC, Walling A, Beck T, Fray M, Peters J, Weaver T, Brown SD, Hancock JM, Mallon AM
    Nucleic Acids Res. 2010;38;D593-9. PMID: 19854936 DOI: 10.1093/nar/gkp867
  • Phenotypic annotation of the mouse X chromosome.
    Cox BJ, Vollmer M, Tamplin O, Lu M, Biechele S, Gertsenstein M, van Campenhout C, Floss T, Kühn R, Wurst W, Lickert H, Rossant J
    Genome Res. 2010;20;1154-64. PMID: 20548051 DOI: 10.1101/gr.105106.110
  • ARCHAEA-ExPRESs targeting of alpha-tubulin 4 mRNA: a model for high-specificity trans-splicing.
    Deidda G, Rossi N, Putti S, Tocchini-Valentini GP
    FASEB J. 2010;24;2976-84. PMID: 20371619 DOI: 10.1096/fj.10-154658
  • A recombineering pipeline to make conditional targeting constructs.
    Fu J, Teucher M, Anastassiadis K, Skarnes W, Stewart AF
    Methods Enzymol. 2010;477;125-44. PMID: 20699140 DOI: 10.1016/S0076-6879(10)77008-7
  • Down syndrome: from understanding the neurobiology to therapy.
    Gardiner K, Herault Y, Lott IT, Antonarakis SE, Reeves RH, Dierssen M
    J Neurosci. 2010;30;14943-5. PMID: 21068296 DOI: 10.1523/JNEUROSCI.3728-10.2010
  • Gene knockdown in the mouse through RNAi.
    Kleinhammer A, Wurst W, Kühn R
    Methods Enzymol. 2010;477;387-414. PMID: 20699152 DOI: 10.1016/S0076-6879(10)77020-8
  • An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase.
    Kranz A, Fu J, Duerschke K, Weidlich S, Naumann R, Stewart AF, Anastassiadis K
    Genesis. 2010;48;512-20. PMID: 20506501 DOI: 10.1002/dvg.20641
  • Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators.
    Landeira D, Sauer S, Poot R, Dvorkina M, Mazzarella L, Jørgensen HF, Pereira CF, Leleu M, Piccolo FM, Spivakov M, Brookes E, Pombo A, Fisher C, Skarnes WC, Snoek T, Bezstarosti K, Demmers J, Klose RJ, Casanova M, Tavares L, Brockdorff N, Merkenschlager M, Fisher AG
    Nat Cell Biol. 2010;12;618-24. PMID: 20473294 DOI: 10.1038/ncb2065
  • Genome-wide forward genetic screens in mouse ES cells.
    Li MA, Pettitt SJ, Yusa K, Bradley A
    Methods Enzymol. 2010;477;217-42. PMID: 20699144 DOI: 10.1016/S0076-6879(10)77012-9
  • Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases.
    Meyer M, de Angelis MH, Wurst W, Kühn R
    Proc Natl Acad Sci U S A. 2010;107;15022-6. PMID: 20686113 DOI: 10.1073/pnas.1009424107
  • EuroPhenome: a repository for high-throughput mouse phenotyping data.
    Morgan H, Beck T, Blake A, Gates H, Adams N, Debouzy G, Leblanc S, Lengger C, Maier H, Melvin D, Meziane H, Richardson D, Wells S, White J, Wood J, The EUMODIC Consortium, de Angelis MH, Brown SD, Hancock JM, Mallon AM
    Nucleic Acids Res. 2010 Jan;38(Database issue):D577-85. PMID: 19933761 DOI: 10.1093/nar/gkp1007
  • Dual RMCE for efficient re-engineering of mouse mutant alleles.
    Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J
    Nat Methods. 2010;7;893-5. PMID: 20953177 DOI: 10.1038/nmeth.1521
  • PiggyBac transposon mutagenesis: a tool for cancer gene discovery in mice.
    Rad R, Rad L, Wang W, Cadinanos J, Vassiliou G, Rice S, Campos LS, Yusa K, Banerjee R, Li MA, de la Rosa J, Strong A, Lu D, Ellis P, Conte N, Yang FT, Liu P, Bradley A
    Science. 2010;330;1104-7. PMID: 20947725 DOI: 10.1126/science.1193004
  • Microphthalmia, parkinsonism, and enhanced nociception in Pitx3 ( 416insG ) mice.
    Rosemann M, Ivashkevich A, Favor J, Dalke C, Hölter SM, Becker L, Rácz I, Bolle I, Klempt M, Rathkolb B, Kalaydjiev S, Adler T, Aguilar A, Hans W, Horsch M, Rozman J, Calzada-Wack J, Kunder S, Naton B, Gailus-Durner V, Fuchs H, Schulz H, Beckers J, Busch DH, Burbach JP, Smidt MP, Quintanilla-Martinez L, Esposito I, Klopstock T, Klingenspor M, Ollert M, Wolf E, Wurst W, Zimmer A, de Angelis MH, Atkinson M, Heinzmann U, Graw J
    Mamm Genome. 2010;21;13-27. PMID: 20033184 DOI: 10.1007/s00335-009-9235-0
  • Efficient conditional and promoter-specific in vivo expression of cDNAs of choice by taking advantage of recombinase-mediated cassette exchange using FlEx gene traps.
    Schebelle L, Wolf C, Stribl C, Javaheri T, Schnütgen F, Ettinger A, Ivics Z, Hansen J, Ruiz P, von Melchner H, Wurst W, Floss T
    Nucleic Acids Res. 2010;38;e106. PMID: 20139417 DOI: 10.1093/nar/gkq044
  • Research funding. Sustaining the data and bioresource commons.
    Schofield PN, Eppig J, Huala E, de Angelis MH, Harvey M, Davidson D, Weaver T, Brown S, Smedley D, Rosenthal N, Schughart K, Aidinis V, Tocchini-Valentini G, Hancock JM
    Science. 2010;330;592-3. PMID: 21030633 DOI: 10.1126/science.1191506
  • CIN85 regulates dopamine receptor endocytosis and governs behaviour in mice.
    Shimokawa N, Haglund K, Hölter SM, Grabbe C, Kirkin V, Koibuchi N, Schultz C, Rozman J, Hoeller D, Qiu CH, Londoño MB, Ikezawa J, Jedlicka P, Stein B, Schwarzacher SW, Wolfer DP, Ehrhardt N, Heuchel R, Nezis I, Brech A, Schmidt MH, Fuchs H, Gailus-Durner V, Klingenspor M, Bogler O, Wurst W, Deller T, de Angelis MH, Dikic I
    EMBO J. 2010;29;2421-32. PMID: 20551902 DOI: 10.1038/emboj.2010.120
  • Floxin, a resource for genetically engineering mouse ESCs.
    Singla V, Hunkapiller J, Santos N, Seol AD, Norman AR, Wakenight P, Skarnes WC, Reiter JF
    Nat Methods. 2010;7;50-2. PMID: 19966808 DOI: 10.1038/nmeth.1406
  • Finding and sharing: new approaches to registries of databases and services for the biomedical sciences.
    Smedley D, Schofield P, Chen CK, Aidinis V, Ainali C, Bard J, Balling R, Birney E, Blake A, Bongcam-Rudloff E, Brookes AJ, Cesareni G, Chandras C, Eppig J, Flicek P, Gkoutos G, Greenaway S, Gruenberger M, Hériché JK, Lyall A, Mallon AM, Muddyman D, Reisinger F, Ringwald M, Rosenthal N, Schughart K, Swertz M, Thorisson GA, Zouberakis M, Hancock JM
    Database (Oxford). 2010;2010;baq014. PMID: 20627863 DOI: 10.1093/database/baq014
  • Structural modeling of histone methyltransferase complex Set1C from Saccharomyces cerevisiae using constraint-based docking.
    Tuukkanen A, Huang B, Henschel A, Stewart F, Schroeder M
    Proteomics. 2010;10;4186-95. PMID: 21046623 DOI: 10.1002/pmic.201000283
  • The use of DNA transposons for cancer gene discovery in mice.
    Vassiliou G, Rad R, Bradley A
    Methods Enzymol. 2010;477;91-106. PMID: 20699138 DOI: 10.1016/S0076-6879(10)77006-3
  • EMMA–mouse mutant resources for the international scientific community.
    Wilkinson P, Sengerova J, Matteoni R, Chen CK, Soulat G, Ureta-Vidal A, Fessele S, Hagn M, Massimi M, Pickford K, Butler RH, Marschall S, Mallon AM, Pickard A, Raspa M, Scavizzi F, Fray M, Larrigaldie V, Leyritz J, Birney E, Tocchini-Valentini GP, Brown S, Herault Y, Montoliu L, de Angelis MH, Smedley D
    Nucleic Acids Res. 2010;38;D570-6. PMID: 19783817 DOI: 10.1093/nar/gkp799
  • Systematic phenotyping of mouse mutants.
    Wurst W, de Angelis MH
    Nat Biotechnol. 2010;28;684-5. PMID: 20622838 DOI: 10.1038/nbt0710-684
  • A mouse model of Down syndrome trisomic for all human chromosome 21 syntenic regions.
    Yu T, Li Z, Jia Z, Clapcote SJ, Liu C, Li S, Asrar S, Pao A, Chen R, Fan N, Carattini-Rivera S, Bechard AR, Spring S, Henkelman RM, Stoica G, Matsui S, Nowak NJ, Roder JC, Chen C, Bradley A, Yu YE
    Hum Mol Genet. 2010;19;2780-91. PMID: 20442137 DOI: 10.1093/hmg/ddq179

2009

  • Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice.
    Anastassiadis K, Fu J, Patsch C, Hu S, Weidlich S, Duerschke K, Buchholz F, Edenhofer F, Stewart AF
    Dis Model Mech. 2009 Sep-Oct ;2;508-15. PMID: 19692579 DOI: 10.1242/dmm.003087
  • Towards better mouse models: enhanced genotypes, systemic phenotyping and envirotype modelling.
    Beckers J, Wurst W, de Angelis MH
    Nat Rev Genet. 2009 Jun;10(6):371-80. PMID: 19434078 DOI: 10.1038/nrg2578
  • Site-specific recombinases for manipulation of the mouse genome.
    Birling MC, Gofflot F, Warot X
    Methods Mol Biol. 2009;561;245-63. PMID: 19504076 DOI: 10.1007/978-1-60327-019-9_16
  • The functional annotation of mammalian genomes: the challenge of phenotyping.
    Brown SD, Wurst W, Kühn R, Hancock JM
    Annu Rev Genet. 2009;43;305-33. PMID: 19689210 DOI: 10.1146/annurev-genet-102108-134143
  • Doxycycline-dependent photoactivated gene expression in eukaryotic systems.
    Cambridge SB, Geissler D, Calegari F, Anastassiadis K, Hasan MT, Stewart AF, Huttner WB, Hagen V, Bonhoeffer T
    Nat Methods. 2009;6;527-31. PMID: 19503080 DOI: 10.1038/nmeth.1340
  • Gene-trap vectors and mutagenesis.
    De-Zolt S, Altschmied J, Ruiz P, von Melchner H, Schnütgen F
    Methods Mol Biol. 2009;530;29-47. PMID: 19266330 DOI: 10.1007/978-1-59745-471-1_3
  • A humanized version of Foxp2 affects cortico-basal ganglia circuits in mice.
    Enard W, Gehre S, Hammerschmidt K, Hölter SM, Blass T, Somel M, Brückner MK, Schreiweis C, Winter C, Sohr R, Becker L, Wiebe V, Nickel B, Giger T, Müller U, Groszer M, Adler T, Aguilar A, Bolle I, Calzada-Wack J, Dalke C, Ehrhardt N, Favor J, Fuchs H, Gailus-Durner V, Hans W, Hölzlwimmer G, Javaheri A, Kalaydjiev S, Kallnik M, Kling E, Kunder S, Mossbrugger I, Naton B, Racz I, Rathkolb B, Rozman J, Schrewe A, Busch DH, Graw J, Ivandic B, Klingenspor M, Klopstock T, Ollert M, Quintanilla-Martinez L, Schulz H, Wolf E, Wurst W, Zimmer A, Fisher SE, Morgenstern R, Arendt T, de Angelis MH, Fischer J, Schwarz J, Pääbo S
    Cell. 2009;137;961-71. PMID: 19490899 DOI: 10.1016/j.cell.2009.03.041
  • Targeting embryonic stem cells.
    Friedel RH
    Methods Mol Biol. 2009;561;185-97. PMID: 19504072 DOI: 10.1007/978-1-60327-019-9_12
  • Transposon-mediated genome manipulation in vertebrates.
    Ivics Z, Li MA, Mátés L, Boeke JD, Nagy A, Bradley A, Izsvák Z
    Nat Methods. 2009;6;415-22. PMID: 19478801 DOI: 10.1038/nmeth.1332
  • Overview on mouse mutagenesis.
    Kühn R, Wurst W
    Methods Mol Biol. 2009;530;1-12. PMID: 19266348 DOI: 10.1007/978-1-59745-471-1_1
  • Construction of gene-targeting vectors by recombineering.
    Lee SC, Wang W, Liu P
    Methods Mol Biol. 2009;530;1-13. PMID: 19266337 DOI: 10.1007/978-1-59745-471-1_2
  • Chromosomal mobilization and reintegration of Sleeping Beauty and PiggyBac transposons.
    Liang Q, Kong J, Stalker J, Bradley A
    Genesis. 2009 Jun;47(6):404-8. PMID: 19391106 DOI: 10.1002/dvg.20508
  • Agouti C57BL/6N embryonic stem cells for mouse genetic resources.
    Pettitt SJ, Liang Q, Rairdan XY, Moran JL, Prosser HM, Beier DR, Lloyd KC, Bradley A, Skarnes WC
    Nat Methods. 2009 Jul;6(7):493-5. PMID: 19525957 DOI: 10.1038/nmeth.1342
  • PRIME importance of pathology expertise.
    Schofield PN, Brown SD, Sundberg JP, Arends M, Warren MV, Dubus P, Ellender M, Fiette L, Rozell B, Quintanilla-Martinez L, Raspa M, Song JY, van der Valk M, McKerlie C
    Nat Biotechnol. 2009;27;24-5. PMID: 19131991 DOI: 10.1038/nbt0109-24
  • Post-publication sharing of data and tools.
    Schofield PN, Bubela T, Weaver T, Portilla L, Brown SD, Hancock JM, Einhorn D, Tocchini-Valentini G, Hrabe de Angelis M, Rosenthal N, CASIMIR Rome Meeting participants
    Nature. 2009;461;171-3. PMID: 19741686 DOI: 10.1038/461171a
  • Identification of protein/DNA contacts with dimethyl sulfate: methylation protection and methylation interference.
    Shaw PE, Stewart AF
    Methods Mol Biol. 2009;543;97-104. PMID: 19378162 DOI: 10.1007/978-1-60327-015-1_8
  • Processing of multiple-intron-containing pretRNA.
    Tocchini-Valentini GD, Fruscoloni P, Tocchini-Valentini GP
    Proc Natl Acad Sci U S A. 2009;106;20246-51. PMID: 19910528 DOI: 10.1073/pnas.0911658106
  • A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells.
    Wang W, Bradley A, Huang Y
    Genome Res. 2009 Apr;19(4):667-73. PMID: 19233961 DOI: 10.1101/gr.085621.108
  • Chromosome Engineering in ES Cells.
    van der Weyden L, Shaw-Smith C, Bradley A
    Methods Mol Biol. 2009;530;1-29. PMID: 19266329 DOI: 10.1007/978-1-59745-471-1_4
  • piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells.
    Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hämäläinen R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A
    Nature. 2009 Apr 9;458(7239):766-70. PMID: 19252478 DOI: 10.1038/nature07863
  • Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon.
    Yusa K, Rad R, Takeda J, Bradley A
    Nat Methods. 2009 May;6(5):363-9. PMID: 19337237 DOI: 10.1038/nmeth.1323

2008

  • Novel caspase-suicide proteins for tamoxifen-inducible apoptosis.
    Chu Y, Senghaas N, Köster RW, Wurst W, Kühn R
    Genesis. 2008;46;530-6. PMID: 18802959 DOI: 10.1002/dvg.20426
  • EUCOMM–the European conditional mouse mutagenesis program.
    Friedel RH, Seisenberger C, Kaloff C, Wurst W
    Brief Funct Genomic Proteomic. 2007;6;180-5. PMID: 17967808 DOI: 10.1093/bfgp/elm022
  • ES cell pluripotency and germ-layer formation require the SWI/SNF chromatin remodeling component BAF250a.
    Gao X, Tate P, Hu P, Tjian R, Skarnes WC, Wang Z
    Proc Natl Acad Sci U S A. 2008;105;6656-61. PMID: 18448678 DOI: 10.1073/pnas.0801802105
  • Extensive genomic copy number variation in embryonic stem cells.
    Liang Q, Conte N, Skarnes WC, Bradley A
    Proc Natl Acad Sci U S A. 2008;105;17453-6. PMID: 18988746 DOI: 10.1073/pnas.0805638105
  • MausDB: an open source application for phenotype data and mouse colony management in large-scale mouse phenotyping projects.
    Maier H, Lengger C, Simic B, Fuchs H, Gailus-Durner V, Hrabé de Angelis M
    BMC Bioinformatics. 2008;9;169. PMID: 18366799 DOI: 10.1186/1471-2105-9-169
  • Analysis of CRE-mediated recombination driven by myosin light chain 1/3 regulatory elements in embryonic and adult skeletal muscle: a tool to study fiber specification.
    Mourkioti F, Slonimsky E, Huth M, Berno V, Rosenthal N
    Genesis. 2008;46;424-30. PMID: 18693277 DOI: 10.1002/dvg.20419
  • Modeling insertional mutagenesis using gene length and expression in murine embryonic stem cells.
    Nord AS, Vranizan K, Tingley W, Zambon AC, Hanspers K, Fong LG, Hu Y, Bacchetti P, Ferrin TE, Babbitt PC, Doniger SW, Skarnes WC, Young SG, Conklin BR
    PLoS ONE. 2007;2;e617. PMID: 17637833 DOI: 10.1371/journal.pone.0000617
  • Deletion of glucose transporter GLUT8 in mice increases locomotor activity.
    Schmidt S, Gawlik V, Hölter SM, Augustin R, Scheepers A, Behrens M, Wurst W, Gailus-Durner V, Fuchs H, Hrabé de Angelis M, Kluge R, Joost HG, Schürmann A
    Behav Genet. 2008;38;396-406. PMID: 18461434 DOI: 10.1007/s10519-008-9208-1
  • Enhanced gene trapping in mouse embryonic stem cells.
    Schnütgen F, Hansen J, De-Zolt S, Horn C, Lutz M, Floss T, Wurst W, Noppinger PR, von Melchner H
    Nucleic Acids Res. 2008;36;e133. PMID: 18812397 DOI: 10.1093/nar/gkn603
  • Pleiotropic effects in Eya3 knockout mice.
    Soker T, Dalke C, Puk O, Floss T, Becker L, Bolle I, Favor J, Hans W, Hoelter SM, Horsch M, Kallnik M, Kling E, Morth C, Schrewe A, Stigloher C, Topp S, Gailus-Durner V, Naton B, Beckers J, Fuchs H, Ivandic B, Klopstock T, Schulz H, Wolf E, Wurst W, Bally-Cuif L, Hrabe de Angelis M, Graw J
    BMC Dev Biol. 2008;8;118. PMID: 19102749 DOI: 10.1186/1471-213X-8-118
  • Simultaneous Cre-mediated conditional knockdown of two genes in mice.
    Steuber-Buchberger P, Wurst W, Kühn R
    Genesis. 2008;46;144-51. PMID: 18327788 DOI: 10.1002/dvg.20376
  • A DNA transposon-based approach to validate oncogenic mutations in the mouse.
    Su Q, Prosser HM, Campos LS, Ortiz M, Nakamura T, Warren M, Dupuy AJ, Jenkins NA, Copeland NG, Bradley A, Liu P
    Proc Natl Acad Sci U S A. 2008;105;19904-9. PMID: 19064922 DOI: 10.1073/pnas.0807785105
  • Chromosomal transposition of PiggyBac in mouse embryonic stem cells.
    Wang W, Lin C, Lu D, Ning Z, Cox T, Melvin D, Wang X, Bradley A, Liu P
    Proc Natl Acad Sci U S A. 2008 Jul 8;105(27):9290-5. PMID: 18579772 DOI: 10.1073/pnas.0801017105

2007

  • A mouse for all reasons.
    International Mouse Knockout Consortium, Collins FS, Rossant J, Wurst W
    Cell. 2007;128;9-13. PMID: 17218247 DOI: 10.1016/j.cell.2006.12.018
  • A new partner for the international knockout mouse consortium.
    Collins FS, Finnell RH, Rossant J, Wurst W
    Cell. 2007;129;235. PMID: 17448981 DOI: 10.1016/j.cell.2007.04.007
  • Hdac2 regulates the cardiac hypertrophic response by modulating Gsk3 beta activity.
    Trivedi CM, Luo Y, Yin Z, Zhang M, Zhu W, Wang T, Floss T, Goettlicher M, Noppinger PR, Wurst W, Ferrari VA, Abrams CS, Gruber PJ, Epstein JA
    Nat Med. 2007;13;324-31. PMID: 17322895 DOI: 10.1038/nm1552

2006

  • The International Gene Trap Consortium Website: a portal to all publicly available gene trap cell lines in mouse.
    Nord AS, Chang PJ, Conklin BR, Cox AV, Harper CA, Hicks GG, Huang CC, Johns SJ, Kawamoto M, Liu S, Meng EC, Morris JH, Rossant J, Ruiz P, Skarnes WC, Soriano P, Stanford WL, Stryke D, von Melchner H, Wurst W, Yamamura K, Young SG, Babbitt PC, Ferrin TE
    Nucleic Acids Res. 2006;34;D642-8. PMID: 16381950 DOI: 10.1093/nar/gkj097
  • Engineering embryonic stem cells with recombinase systems.
    Schnütgen F, Stewart AF, von Melchner H, Anastassiadis K
    Methods Enzymol. 2006;420;100-36. PMID: 17161696 DOI: 10.1016/S0076-6879(06)20007-7

2005

  • EMPReSS: standardized phenotype screens for functional annotation of the mouse genome.
    Brown SD, Chambon P, de Angelis MH, 
    Nat Genet. 2005;37;1155. PMID: 16254554 DOI: 10.1038/ng1105-1155
  • Genomewide production of multipurpose alleles for the functional analysis of the mouse genome.
    Schnütgen F, De-Zolt S, Van Sloun P, Hollatz M, Floss T, Hansen J, Altschmied J, Seisenberger C, Ghyselinck NB, Ruiz P, Chambon P, Wurst W, von Melchner H
    Proc Natl Acad Sci U S A. 2005;102;7221-6. PMID: 15870191 DOI: 10.1073/pnas.0502273102

2004

  • The knockout mouse project.
    Austin CP, Battey JF, Bradley A, Bucan M, Capecchi M, Collins FS, Dove WF, Duyk G, Dymecki S, Eppig JT, Grieder FB, Heintz N, Hicks G, Insel TR, Joyner A, Koller BH, Lloyd KC, Magnuson T, Moore MW, Nagy A, Pollock JD, Roses AD, Sands AT, Seed B, Skarnes WC, Snoddy J, Soriano P, Stewart DJ, Stewart F, Stillman B, Varmus H, Varticovski L, Verma IM, Vogt TF, von Melchner H, Witkowski J, Woychik RP, Wurst W, Yancopoulos GD, Young SG, Zambrowicz B
    Nat Genet. 2004;36;921-4. PMID: 15340423 DOI: 10.1038/ng0904-921
  • The European dimension for the mouse genome mutagenesis program.
    Auwerx J, Avner P, Baldock R, Ballabio A, Balling R, Barbacid M, Berns A, Bradley A, Brown S, Carmeliet P, Chambon P, Cox R, Davidson D, Davies K, Duboule D, Forejt J, Granucci F, Hastie N, de Angelis MH, Jackson I, Kioussis D, Kollias G, Lathrop M, Lendahl U, Malumbres M, von Melchner H, Müller W, Partanen J, Ricciardi-Castagnoli P, Rigby P, Rosen B, Rosenthal N, Skarnes B, Stewart AF, Thornton J, Tocchini-Valentini G, Wagner E, Wahli W, Wurst W
    Nat Genet. 2004;36;925-7. PMID: 15340424 DOI: 10.1038/ng0904-925
  • A public gene trap resource for mouse functional genomics.
    Skarnes WC, von Melchner H, Wurst W, Hicks G, Nord AS, Cox T, Young SG, Ruiz P, Soriano P, Tessier-Lavigne M, Conklin BR, Stanford WL, Rossant J, 
    Nat Genet. 2004;36;543-4. PMID: 15167922 DOI: 10.1038/ng0604-543
  • Von Melchner H, Stewart AF. 2004. Engineering of ES cell genomes with recombinase systems.
    In: Handbook of Stem Cells, ed. R Lanza, pp. 609-22. Elsevier Academic Press.

2003

  • A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome.
    Hansen J, Floss T, Van Sloun P, Füchtbauer EM, Vauti F, Arnold HH, Schnütgen F, Wurst W, von Melchner H, Ruiz P
    Proc Natl Acad Sci U S A. 2003;100;9918-22. PMID: 12904583 DOI: 10.1073/pnas.1633296100

2001

  • Functional analysis of secreted and transmembrane proteins critical to mouse development.
    Mitchell KJ, Pinson KI, Kelly OG, Brennan J, Zupicich J, Scherz P, Leighton PA, Goodrich LV, Lu X, Avery BJ, Tate P, Dill K, Pangilinan E, Wakenight P, Tessier-Lavigne M, Skarnes WC
    Nat Genet. 2001;28;241-9. PMID: 11431694 DOI: 10.1038/90074

2000

  • An LDL-receptor-related protein mediates Wnt signalling in mice.
    Pinson KI, Brennan J, Monkley S, Avery BJ, Skarnes WC
    Nature. 2000;407;535-8. PMID: 11029008 DOI: 10.1038/35035124
  • Gene trapping methods for the identification and functional analysis of cell surface proteins in mice.
    Skarnes WC
    Methods Enzymol. 2000;328;592-615. PMID: 11075368

1997

  • Rapid sequence analysis of gene trap integrations to generate a resource of insertional mutations in mice.
    Townley DJ, Avery BJ, Rosen B, Skarnes WC
    Genome Res. 1997;7;293-8. PMID: 9074932 DOI: 10.1101/gr.7.3.293

1995

  • Capturing genes encoding membrane and secreted proteins important for mouse development.
    Skarnes WC, Moss JE, Hurtley SM, Beddington RS
    Proc Natl Acad Sci U S A. 1995;92;6592-6. PMID: 7604039

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